Bilirubin is the major bile pigment in the body. There are crucial precautions you should observe in performing bilirubin tests because the substance can be affected by many factors. Here are precautions that are observed in the Jendrassik and Grof method and Evelyn and Malloy method.
Avoid hemolysis of blood specimen.
Bilirubin is a degradation product of Red Blood Cell or RBC, so any lysis or rupture of these blood cells will increase inaccurately the results of the bilirubin test.
Avoid exposure to light.
Biluribin is photosensitive. It is easily destroyed by light, so exposing it to natural or artificial light will erroneously decrease its laboratory results.
Maintain the correct pH.
Maintaining the correct pH or alkalinity and acidity of the test solution and reagent will ensure the reliability of results. This is vital precaution in performing bilirubin tests.
Avoid interference from proteins and foreign substances.
Protein and foreign substances will greatly affect you bilirubin tests. Be sure to cover your solutions at all times with a rubber stopper or a parafilm. This will ensure that the results of your bilirubin tests are accurate and precise.